comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases眼科炎性免疫介质视网膜疾病的综合分析.pdfVIP

Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases 1 1 1 1 1 Takeru Yoshimura , Koh-Hei Sonoda *, Mika Sugahara , Yasutaka Mochizuki , Hiroshi Enaida , Yuji 1 1 1 2 1 Oshima , Akifumi Ueno , Yasuaki Hata , Hiroki Yoshida , Tatsuro Ishibashi 1 Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan, 2 Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Nabeshima, Saga, Japan Abstract Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a total of 83 patients with either idiopathic macular hole (MH) or idiopathic epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, such as ischemic retina and proliferative membranes. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with