原创力文档comprehensive gene expression analysis of human embryonic stem cells during differentiation into neural cells全面的基因表达分析的人类胚胎干细胞分化为神经细胞.pdfVIP
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comprehensive gene expression analysis of human embryonic stem cells during differentiation into neural cells全面的基因表达分析的人类胚胎干细胞分化为神经细胞
Comprehensive Gene Expression Analysis of Human
Embryonic Stem Cells during Differentiation into Neural
Cells
1 2 1,3 1,3 2
Ali Fathi , Maryam Hatami , Vahid Hajihosseini , Faranak Fattahi , Sahar Kiani , Hossein
Baharvand1,4*, Ghasem Hosseini Salekdeh 1,5*
1 Department of Molecular Systems Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, Avicenna Research Institute (ACECR),
Tehran, Iran, 2 Department of Stem Cells and Developmental Biology, Cell Science Research Centre, Royan Institute for Stem Cell Biology and Technology, Avicenna
Research Institute (ACECR), Tehran, Iran, 3 Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran, 4 Department of Developmental Biology,
University of Science and Culture, Avicenna Research Institute (ACECR), Tehran, Iran, 5 Department of Systems Biology, Agricultural Biotechnology Research Institute of
Iran, Karaj, Iran
Abstract
Global gene expression analysis of human embryonic stem cells (hESCs) that differentiate into neural cells would help to
further define the molecular mechanisms involved in neurogenesis in humans. We performed a comprehensive
transcripteome analysis of hESC differentiation at three different stages: early neural differentiation, neural ectoderm, and
differentiated neurons. We identified and validated time-dependent gene expression patterns and showed that the gene
expression patterns reflect early ESC differentiation. Sets of genes are induced in primary ectodermal lineages and then in
differentiated neurons, constituting consecutive waves of known and novel genes. Pathway analysis revealed dynamic
expression patterns of members of several signaling
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