crispr typing and subtyping for improved laboratory surveillance of salmonella infectionscrispr改善实验室监测的类型和子类型沙门氏菌感染.pdfVIP

CRISPR Typing and Subtyping for Improved Laboratory Surveillance of Salmonella Infections ¨ 1 2 3 1 ´ 1 Laetitia Fabre , Jian Zhang , Ghislaine Guigon , Simon Le Hello , Veronique Guibert , Marie Accou- 1 ¨ 1 1 1 3 3 Demartin , Saıana de Romans , Catherine Lim , Chrystelle Roux , Virginie Passet , Laure Diancourt , Martine Guibourdenche1, Sylvie Issenhuth-Jeanjean 1, Mark Achtman4, Sylvain Brisse3, Christophe Sola2, Franc¸ois-Xavier Weill1* ´ ´ ` ´ ´ 1 Institut Pasteur, Unite des Bacteries Pathogenes Enteriques, Paris, France, 2 Institute of Genetics and Microbiology, UMR8621, IGEPE Team, Universud, CNRS Universite Paris-Sud 11, Orsay, France, 3 Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France, 4 Environmental Research Institute, University College Cork, Cork, Ireland Abstract Laboratory surveillance systems for salmonellosis should ideally be based on the rapid serotyping and subtyping of isolates. However, current typing methods are limited in both speed and precision. Using 783 strains and isolates belonging to 130 serotypes, we show here that a new family of DNA repeats named CRISPR (clustered regularly interspaced short palindromic repeats) is highly polymorphic in Salmonella. We found that CRISPR polymorphism was strongly correlated with both serotype and multilocus sequence type. Furthermore, spacer m