cyanobacterial cell lineage analysis of the spatiotemporal hetr expression profile during heterocyst pattern formation in anabaena sp. pcc 7120蓝藻的细胞谱系分析时空hetr异形细胞中表达谱模式形成的项圈藻pcc 7120 sp.pdfVIP

Cyanobacterial Cell Lineage Analysis of the Spatiotemporal hetR Expression Profile during Heterocyst Pattern Formation in Anabaena sp. PCC 7120 1. 1. 3 4 1 Hironori Asai , Shunsuke Iwamori , Kentaro Kawai , Shigeki Ehira , Jun-ichi Ishihara , Kazuyuki Aihara5,6, Shuichi Shoji1,3, Hideo Iwasaki1,2.* 1 Department of Electrical Engineering and Biological Science, Waseda University (TWIns), Tokyo, Japan, 2 PRESTO, Japan Science and Technology Agency (JST), Tokyo, Japan, 3 Department of Electrocic and Photonic Systems, Waseda University, Tokyo, Japan, 4 Department of Biological Science, Chuo University, Kasuga, Tokyo, 5 Institute of Industrial Science, The University of Tokyo, Tokyo, Japan, 6 ERATO Aihara Complexity Modelling Project, JST, Tokyo, Japan Abstract Diazotrophic heterocyst formation in the filamentous cyanobacterium, Anabaena sp. PCC 7120, is one of the simplest pattern formations known to occur in cell differentiation. Most previous studies on heterocyst patterning were based on statistical analysis using cells collected or observed at different times from a liquid culture, which would mask stochastic fluctuations affecting the process of pattern formation dynamics in a single bacterial filament. In order to analyze the spatiotemporal dynamics of heterocyst formation at the single filament level, here we developed a culture system to monitor simultaneously bacterial development, gene expression, and phycobilisome fluorescence. We also developed micro-liquid chamber arrays to analyze multiple Anabaena filaments at the same time. Cell lineage analyses demonstrated that the initial distributions of hetR::gfp and phycobilisome fluorescence signals at nitrogen step-do