de novo assembly of the complete genome of an enhanced electricity-producing variant of geobacter sulfurreducens using only short reads新创的完整基因组的组装核废料旁边的一个增强的发电变体sulfurreducens只使用短的读取.pdfVIP

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de novo assembly of the complete genome of an enhanced electricity-producing variant of geobacter sulfurreducens using only short reads新创的完整基因组的组装核废料旁边的一个增强的发电变体sulfurreducens只使用短的读取.pdf

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de novo assembly of the complete genome of an enhanced electricity-producing variant of geobacter sulfurreducens using only short reads新创的完整基因组的组装核废料旁边的一个增强的发电变体sulfurreducens只使用短的读取

De Novo Assembly of the Complete Genome of an Enhanced Electricity-Producing Variant of Geobacter sulfurreducens Using Only Short Reads 1,2 3 3¤ 2 2 3 Harish Nagarajan , Jessica E. Butler , Anna Klimes , Yu Qiu , Karsten Zengler , Joy Ward , Nelson D. 3 ´4 2 3 2 Young , Barbara A. Methe , Bernhard Ø. Palsson , Derek R. Lovley , Christian L. Barrett * 1 Bioinformatics and Systems Biology Graduate Program, University of California San Diego, La Jolla, California, United States of America, 2 Department of Bioengineering, University of California San Diego, La Jolla, California, United States of America, 3 Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, United States of America, 4 Department of Microbial and Environmental Genomics, J. Craig Venter Institute, Rockville, Maryland, United States of America Abstract State-of-the-art DNA sequencing technologies are transforming the life sciences due to their ability to generate nucleotide sequence information with a speed and quantity that is unapproachable with traditional Sanger sequencing. Genome sequencing is a principal application of this technology, where the ultimate goal is the full and complete sequence of the organism of interest. Due to the nature of the raw data produced by these technologies, a full genomic sequence attained without the aid of Sanger sequencing has yet to be demonstrated. We have successfully developed a four-phase strategy for using only next-generation sequencing technologies (Illumina and 454) to assemble a complete microbial genome de novo. We applied this