de novo sequencing of hypericum perforatum transcriptome to identify potential genes involved in the biosynthesis of active metabolites贯叶连翘的从头测序转录组来识别潜在的基因活性代谢产物的生物合成.pdfVIP

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de novo sequencing of hypericum perforatum transcriptome to identify potential genes involved in the biosynthesis of active metabolites贯叶连翘的从头测序转录组来识别潜在的基因活性代谢产物的生物合成.pdf

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de novo sequencing of hypericum perforatum transcriptome to identify potential genes involved in the biosynthesis of active metabolites贯叶连翘的从头测序转录组来识别潜在的基因活性代谢产物的生物合成

De Novo Sequencing of Hypericum perforatum Transcriptome to Identify Potential Genes Involved in the Biosynthesis of Active Metabolites 1 2 3 1 1 Miao He , Ying Wang , Wenping Hua , Yuan Zhang , Zhezhi Wang * 1 Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an, Shaanxi, People’s Republic of China, 2 Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Institute of Life Science, Northwest University, Xi’an, Shaanxi, People’s Republic of China, 3 Department of Life Sciences, Shaanxi Institute of Education, Xi’an, Shaanxi, People’s Republic of China Abstract Background: Hypericum perforatum L. (St. John’s wort) is a medicinal plant with pharmacological properties that are antidepressant, anti-inflammatory, antiviral, anti-cancer, and antibacterial. Its major active metabolites are hypericins, hyperforins, and melatonin. However, little genetic information is available for this species, especially that concerning the biosynthetic pathways for active ingredients. Methodology/Principal Findings: Using de novo transcriptome analysis, we obtained 59,184 unigenes covering the entire life cycle of these plants. In all, 40,813 unigenes (68.86%) were annotated and 2,359 were assigned to secondary metabolic pathways. Among them, 260 unigenes are involved in the production of hypericin, hyperforin, and melatonin. Another 2,291 unigenes are classified as potential Type III polyketide synthase. Our BlastX search against the AGRIS database reveals 1,772 unigenes that are h