definition of the σw regulon of bacillus subtilis in the absence of stress定义σw调节子的枯草芽孢杆菌在没有压力.pdfVIP

Definition of the sW Regulon of Bacillus subtilis in the Absence of Stress 1 2 3 1 1 Jessica C. Zweers , Pierre Nicolas , Thomas Wiegert , Jan Maarten van Dijl *, Emma L. Denham ´ 1 Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands, 2 INRA, UR1077, Mathematique ´ ¨ Informatique et Genome, Jouy-en-Josas, France, 3 Hochschule Zittau/Gorlitz, FN/Biotechnologie, Zittau, Germany Abstract Bacteria employ extracytoplasmic function (ECF) sigma factors for their responses to environmental stresses. Despite intensive research, the molecular dissection of ECF sigma factor regulons has remained a major challenge due to overlaps in the ECF sigma factor-regulated genes and the stimuli that activate the different ECF sigma factors. Here we have employed tiling arrays to single out the ECF sW X regulon of the Gram-positive bacterium Bacillus subtilis from the overlapping ECF s , Y M s , and s regulons. For this purpose, we profiled the transcriptome of a B. subtilis sigW mutant under non-stress conditions to select candidate genes that are strictly sW-regulated. Under these conditions, sW exhibits a basal level of activity. Subsequently,