cross-reacting antibacterial auto-antibodies are produced within coronary atherosclerotic plaques of acute coronary syndrome patients交叉反应生产抗菌auto-antibodies在急性冠脉综合征患者的冠状动脉粥样硬化性斑块.pdfVIP

Cross-Reacting Antibacterial Auto-Antibodies Are Produced within Coronary Atherosclerotic Plaques of Acute Coronary Syndrome Patients Filippo Canducci1,2*, Diego Saita1,2, Chiara Foglieni2,3, Maria Rosaria Piscopiello2,4, Roberto Chiesa2,5, 2,6 2,3 7 1,2 1,2 Antonio Colombo , Domenico Cianflone , Attilio Maseri , Massimo Clementi , Roberto Burioni * 1 Laboratory of Microbiology and Virology, Ospedale San Raffaele, Milan, Italy, 2 University Vita-Salute San Raffaele, Milan, Italy, 3 Clinical Cardiovascular Biology Centre, Ospedale San Raffaele, Milan, Italy, 4 Istituto di Neurologia Sperimentale, Ospedale San Raffaele, Milan, Italy, 5 Division of Vascular Surgery, Ospedale San Raffaele, Milan, Italy, 6 Interventional Cardiology Unit, Department of Cardio-Thoracic and Vascular Diseases, Ospedale San Raffaele, Milan Italy, 7 Heart Care Foundation, ONLUS, Florence, Italy Abstract Coronary atherosclerosis, the main condition predisposing to acute myocardial infarction, has an inflammatory component caused by stimuli that are yet unknown. We molecularly investigated the nature of the immune response within human coronary lesion in four coronary plaques obtained by endoluminal atherectomy from four patients. We constructed phage- display libraries containing the IgG1/kappa antibody fragments produced by B-lymphocytes present in each plaque. By immunoaffinity, we selected from these libraries a monoclonal antibody, arbitrarily named Fab7816, able to react both with coronary and carotid atherosclerotic tissue samples. We also demonstrated by confocal microscopy that this monoclonal antibody recognized human transgelin type 1, a cytoskeleton protein involved in atherogenesis, and that it co-l