computational and statistical analysis of protein mass spectrometry data计算和统计分析的蛋白质质谱数据.pdfVIP

Review Computational and Statistical Analysis of Protein Mass Spectrometry Data William Stafford Noble1,2*, Michael J. MacCoss1 1 Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America, 2 Department of Computer Science and Engineering, University of Washington, Seattle, Washington, United States of America addition, intellectual property issues, such as the SEQUEST Abstract: High-throughput proteomics experiments in- patents held by the University of Washington, may have volving tandem mass spectrometry produce large vol- discouraged some researchers from entering the field. umes of complex data that require sophisticated compu- An equally important impediment, however, is the ‘‘energy tational analyses. As such, the field offers many challenges barrier’’ associated with starting out in mass spectrometry. In the for computational biologists. In this article, we briefly late 1990s, microarray analysis took off with surprising rapidity, in introduce some of the core computational and statistical part because the data could be fairly accurately summarized in problems in the field and then describe a variety of outstanding problems that readers of PLoS Computational matrix format, and manipulating matrices is familiar to computer Biology might be able to help solve. scientistics, electrical engineers, and physicists. This is not the case