denitrification performance and microbial community structure of a combined wla–obco system脱氮性能和微生物群落的结构结合wla-obco系统.pdfVIP

Denitrification Performance and Microbial Community Structure of a Combined WLA–OBCO System 1 2 1 1 1 Tinglin Huang *, Wei Wei , Junfeng Su , Haihan Zhang , Na Li 1 School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an, Shaanxi, China, 2 Tianjin Waterworks Group Co. Ltd., Tianjin, China Abstract The contamination of surface water by nitrogen due to fertilizer application and discharge of wastewater is an increasingly serious problem. A multifunctional device, which combines water-lifting and aeration (WLA) with oligotrophic biological contact oxidation (OBCO), was developed for pretreatment of raw water to reduce nitrogen. The performance of nitrogen removal and changes in microbial community structure were investigated. The results showed that the combined technique of WLA-OBCO was feasible, and that ammonium, nitrate, total nitrogen and total organic carbon were effectively removed. Meanwhile, nitrite was mostly undetectable. The PCR-DGGE and clone sequencing results revealed that a-proteobacterium was the largest bacterial group, and Pseudomonas strains Y3 and J8 were the dominant bacteria. Citation: Huang T, Wei W, Su J, Zhang H, Li N (2012) Denitrification Performance and Microbial Community Structure of a Combined WLA–OBCO System. PLoS ONE 7(11): e48339. doi:10.1371/journal.pone.0048339 Editor: Jack Anthony Gilbert, Argonne National Laboratory, United States of America Received June 11, 2012; Accepted September 24, 2012; Published November 9, 2012 Copyright: 2012 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.