density contrast sedimentation velocity for the determination of protein partial-specific volumes密度反差沉降速度测定的蛋白质partial-specific卷.pdfVIP

Density Contrast Sedimentation Velocity for the Determination of Protein Partial-Specific Volumes 1 1 2 3,4,5 2 Patrick H. Brown , Andrea Balbo , Huaying Zhao , Christine Ebel , Peter Schuck * 1 Biomedical Engineering and Physical Sciences Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Dynamics of Macromolecular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of ´ Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Institut de Biologie Structurale, Universite Grenoble ` 1, Grenoble, France, 4 Centre National de la Recherche Scientifique, Grenoble, France, 5 Commisariat a l’Energie Atomique, Grenoble, France Abstract The partial-specific volume of proteins is an important thermodynamic parameter required for the interpretation of data in several biophysical disciplines. Building on recent advances in the use of density variation sedimentation velocity analytical ultracentrifugation for the determination of macromolecular partial-specific volumes, we have explored a direct global modeling approach describing the sedimentation boundaries in different solvents with a joint differential sedimentation coefficient distribution. This takes full advantage of the influence of different macromolecular buoyancy on both the spread and the velocity of the sedimenta