determination of cellular lipids bound to human cd1d molecules测定细胞脂质结合人类cd1d分子.pdfVIP

Determination of Cellular Lipids Bound to Human CD1d Molecules 1,3 2 1 1 1 1 Daryl Cox , Lisa Fox , Runying Tian , Wilfried Bardet , Matthew Skaley , Danijela Mojsilovic , Jenny Gumperz2.*, William Hildebrand 1. 1 Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States of America, 2 Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America, 3 Department of Chemistry, Southern Nazarene University, Bethany, Oklahoma, United States of America Abstract CD1 molecules are glycoproteins that present lipid antigens at the cell surface for immunological recognition by specialized populations of T lymphocytes. Prior experimental data suggest a wide variety of lipid species can bind to CD1 molecules, but little is known about the characteristics of cellular ligands that are selected for presentation. Here we have molecularly characterized lipids bound to the human CD1d isoform. Ligands were eluted from secreted CD1d molecules and separated by normal phase HPLC, then characterized by mass spectroscopy. A total of 177 lipid species were molecularly identified, comprising glycerophospholipids and sphingolipids. The glycerophospholipids included common diacylglycerol species, reduced forms known as plasmalogens, lyso-phospholipids (monoacyl species), and cardiolipins (tetraacyl species). The sphingolipids included sphingomyelins and glycosylated forms, such as the ganglioside GM3. These results demonstrate that human CD1d molecules bind a surprising diversity of lipid structures within the secretory pathway, including compounds that