decreased circulating endothelial progenitor cell levels and function in patients with nonalcoholic fatty liver disease减少循环内皮祖细胞水平和非酒精性脂肪肝患者的功能.pdfVIP

Decreased Circulating Endothelial Progenitor Cell Levels and Function in Patients with Nonalcoholic Fatty Liver Disease Chia-Hung Chiang1,5, Po-Hsun Huang1,4,5*, Fa-Po Chung1,5, Zu-Yin Chen1,5, Hsin-Bang Leu1,3,4,5, Chin-Chou Huang1,2,5,6, Tao-Cheng Wu1,4,5, Jaw-Wen Chen1,2,5,6, Shing-Jong Lin1,2,4,5 1 Division of Cardiology, Taipei Veterans General Hospital, Taipei, Taiwan, 2 Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan, 3 Healthcare and Management Center, Taipei Veterans General Hospital, Taipei, Taiwan, 4 Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, 5 Cardiovascular Research Center, National Yang-Ming University, Taipei, Taiwan, 6 Institute and Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan Abstract Objectives: Nonalcoholic fatty liver disease (NAFLD) is associated with advanced atherosclerosis and a higher risk of cardiovascular disease. Increasing evidence suggests that injured endothelial monolayer is regenerated by circulating bone marrow derived-endothelial progenitor cells (EPCs), and levels of circulating EPCs reflect vascular repair capacity. However, the relation between NAFLD and EPC remains unclear. Here, we tested the hypothesis that patients with nonalcoholic fatty liver disease (NAFLD) might have decreased endothelial progenitor cell (EPC) levels and attenuated EPC function. Methods and Results: A total of 312 consecutive patients undergoing elective coronary angiography because of suspected coronary artery disease were screened and received examinations of abdominal ultrasonography between July 2009 and November 2010. Finally, 34 patients with an ultrasonographic diagnosis of NAFLD, and 68 age- and sex-matched controls without NAFLD were enrolled. Flow cytometry with quantification of EPC markers (defined as CD34+