differential proteomic analysis of arabidopsis thaliana genotypes exhibiting resistance or susceptibility to the insect herbivore, plutella xylostella差异蛋白质组学分析的拟南芥基因型表现出阻力或易感性昆虫食草动物,小菜蛾.pdfVIP

Differential Proteomic Analysis of Arabidopsis thaliana Genotypes Exhibiting Resistance or Susceptibility to the Insect Herbivore, Plutella xylostella 1 1 1 1 2 Richard M. Collins *, Muhammed Afzal , Deborah A. Ward , Mark C. Prescott , Steven M. Sait , Huw H. Rees1, A. Brian Tomsett1 1 School of Biological Sciences, The University of Liverpool, Liverpool, United Kingdom, 2 Institute of Integrative and Comparative Biology, University of Leeds, Leeds, United Kingdom Abstract A proteomic study was conducted to investigate physiological factors affecting feeding behaviour by larvae of the insect, Plutella xylostella, on herbivore-susceptible and herbivore-resistant Arabidopsis thaliana. The leaves of 162 recombinant inbred lines (Rils) were screened to detect genotypes upon which Plutella larvae fed least (P. xylostella-resistant) or most (P. xylostella-susceptible). 2Drevealed significant differences in the proteomes between the identified resistant and susceptible Rils. The proteomic results, together with detection of increased production of hydrogen peroxide in resistant Rils, suggest a correlation between P. xylostella resistance and the production of increased levels of reactive oxygen species (ROS), in particular H O , and that this was expressed prior to herbivory. Many of the proteins that were more abundant in 2 2 the Plutella-resistant Rils are known in other biological systems to be involved in limiting ROS damage. Such proteins included carbonic anhydrases, malate dehydrogenases, glutathione S-transferases, isocitrate dehydrogenase-like protein (R1), and lipoamide dehydrogenase. In addition, patterns of germin-like protein 3 isoforms could also be indicative o