differential proteomic analysis of platelets suggested possible signal cascades network in platelets treated with salvianolic acid b差异蛋白质组学分析血小板血小板治疗建议可能的信号级联网络salvianolic酸b.pdfVIP

Differential Proteomic Analysis of Platelets Suggested Possible Signal Cascades Network in Platelets Treated with Salvianolic Acid B 1 1 1 2 2 1 1 Chao Ma , Yan Yao , Qing-Xi Yue , Xin-Wen Zhou , Peng-Yuan Yang , Wan-Ying Wu , Shu-Hong Guan , 1 1 1 1 Bao-Hong Jiang , Min Yang , Xuan Liu *, De-An Guo * 1 Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, People’s Republic of China, 2 Institutes of Biomedical Sciences, Fudan University, Shanghai, People’s Republic of China Abstract Background: Salvianolic acid B (SB) is an active component isolated from Danshen, a traditional Chinese medicine widely used for the treatment of cardiovascular disorders. Previous study suggested that SB might inhibit adhesion as well as aggregation of platelets by a mechanism involving the integrin a2b1. But, the signal cascades in platelets after SB binding are still not clear. Methodology/Principal Findings: In the present study, a differential proteomic analysis (two-dimensional electrophoresis) was conducted to check the protein expression profiles of rat platelets with or without treatment of SB. Proteins altered in level after SB exposure were identified by MALDI-TOF MS/MS. Treatment of SB caused regulation of 20 proteins such as heat shock-related 70 kDa protein 2 (hsp70), LIM domain protein CLP-36, copine I, peroxiredoxin-2, coronin-1 B and cytoplasmic dynein intermediate chain 2C. The regulation of SB on protein levels was confirmed by Western blotting. The signal cascades network induced by SB after its binding with integrin a2