differential regulation and recovery of intracellular ca2+ in cerebral and small mesenteric arterial smooth muscle cells of simulated microgravity rat微分调节和恢复大脑的细胞内钙离子和小肠系膜动脉平滑肌细胞模拟微重力的老鼠.pdfVIP

Differential Regulation and Recovery of Intracellular Ca2+ in Cerebral and Small Mesenteric Arterial Smooth Muscle Cells of Simulated Microgravity Rat 1. 2. 3. 3 4 4 Jun-Hui Xue , Lian-Hong Chen , Hua-Zhou Zhao , Yong-Dong Pu , Han-Zhong Feng , Yu-Guang Ma , 4 1,4 1 4 Jin Ma , Yao-Ming Chang , Zuo-Ming Zhang *, Man-Jiang Xie * 1 Department of Aerospace Clinical Medicine, Key Laboratory of Aerospace Medicine of Ministry of Education, Fourth Military Medical University, Xi’an, Shaanxi Province, China, 2 Department of Chest Surgery, Tangdu Hospital of Fourth Military Medical University, Xi’an, Shaanxi Province, China, 3 309 Clinical Divisions, Department of General Surgery, General Hospital of PLA, Beijing, China, 4 Department of Aerospace Physiology, Key Laboratory of Aerospace Medicine of Ministry of Education, Fourth Military Medical University, Xi’an, Shaanxi Province, China Abstract Background: The differential adaptations of cerebrovasculature and small mesenteric arteries could be one of critical factors in postspaceflight orthostatic intolerance, but the cellular mechanisms remain unknown. We hypothesize that there is a differential regulation of intracellular Ca2+ determined by the alterations in the functions of plasma membrane CaL channels and ryanodine-sensitive Ca2+ releases from sarcoplasmic reticulum (SR) in cerebral and small mesenteric vascular smooth muscle cells (VSMCs) of simulated microgravity rats, respectively. Methodology/Principal Findings: Sprague-Dawley rats were subjected to 28-day hindlimb unweighting to simulate micro