differential requirements of two reca mutants for constitutive sos expression in escherichia coli k-12微分的要求两个reca突变体本构sos表达大肠杆菌k - 12.pdfVIP

Differential Requirements of Two recA Mutants for Constitutive SOS Expression in Escherichia coli K-12 1 2 2 1,2 Jarukit Edward Long , Nicholas Renzette , Richard C. Centore , Steven J. Sandler * 1 Department of Microbiology, Morrill Science Center IV N203, University of Massachusetts at Amherst, Amherst, Massachusetts, United States of America, 2 Molecular and Cellular Biology Graduate Program, Morrill Science Center, University of Massachusetts at Amherst, Amherst, Massachusetts, United States of America Abstract Background: Repairing DNA damage begins with its detection and is often followed by elicitation of a cellular response. In E. coli, RecA polymerizes on ssDNA produced after DNA damage and induces the SOS Response. The RecA-DNA filament is an allosteric effector of LexA auto-proteolysis. LexA is the repressor of the SOS Response. Not all RecA-DNA filaments, however, lead to an SOS Response. Certain recA mutants express the SOS Response (recAC) in the absence of external DNA damage in log phase cells. Methodology/Principal Findings: Genetic analysis of two recAC mutants was used to determine the mechanism of constitutive SOS (SOSC) expression in a population of log phase cells using fluorescence of single cells carrying an SOS reporter system (sulAp-gfp). SOSC expression in recA4142 mutants was dependent on its initial level of transcription, recBCD, recFOR, recX, dinI, xthA and the type of medium in which the cells were grown. SOSC expression in recA730 mutants was affected by none of the mutations or conditions tested above. Conclusions/Significance: It is concluded that not all recAC alleles cause SOSC exp