differential transcription of bacteriophage φx174 genes at 37°c and 42°c微分的噬菌体φx174基因转录37°c和42°c.pdfVIP

Differential Transcription of Bacteriophage wX174 Genes at 37 C and 42 C 6 6 Luyi Zhao, Amber D. Stancik, Celeste J. Brown* Department of Biological Sciences, University of Idaho, Moscow, Idaho, United States of America Abstract To investigate how high temperature affects viral transcription, the absolute amounts of mRNA for six bacteriophage wX174 genes were compared at 37uC and 42uC using Q-PCR. At 37uC, mRNA levels for all genes were consistent with previous studies, but at 42uC mRNA levels for four genes were significantly different from levels at 37uC. Transcript levels were higher for genes B and D; the promoter before gene B appears to be up-regulated at high temperature. Levels for genes F and G were reduced at high temperature, possibly due to increased efficiency of the transcription termination signal immediately upstream of gene F. These functional changes in wX174 gene regulation at high temperature have not been described previously. Studies of phage evolution at high temperatures indicate that this difference in transcript levels is subject to adaptation. Citation: Zhao L, Stancik AD, Brown CJ (2012) Differential Transcription of Bacteriophage wX174 Genes at 37uC and 42uC. PLoS ONE 7(4): e35909. doi:10.1371/ journal.pone.0035909 Editor: Baochuan Lin, Naval Research Laboratory, United States of America Received January 5, 2012; Accepted March 26, 2012; Published April 23, 2012 Copyright: 2012 Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by National Institutes of Health () grant NCRR P20RR16448. The funder had no role in study desig