development of a non-invasive method, multiplex methylation specific pcr (mmsp), for early diagnosis of nasopharyngeal carcinoma非侵入性方法的发展,多路复用甲基化特异性pcr(mmsp),鼻咽癌的早期诊断.pdfVIP

Development of a Non-Invasive Method, Multiplex Methylation Specific PCR (MMSP), for Early Diagnosis of Nasopharyngeal Carcinoma 1,2. 1,2. 3 1 1,5 2 Zhe Zhang , Di Sun , Susanna Hilda Hutajulu , Imran Nawaz , Do Nguyen Van , Guangwu Huang , 3 4 1 1 Sofia M. Haryana , Jaap M. Middeldorp , Ingemar Ernberg , Li-Fu Hu * 1 Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden, 2 Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China, 3 Molecular Biology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 4 Department of Pathology, VU Medical Center, Amsterdam, The Netherlands, 5 Department of Immunology and Pathophysiology, Hanoi Medical University (HMU), Dongda, Hanoi, Vietnam Abstract Increasing evidence demonstrated that inactivation of tumor suppressor genes (TSGs) by aberrant promoter methylation is an early event during carcinogenesis. Aiming at developing early diagnostic or prognostic tools for various tumors, we took an EBV-associated tumor, nasopharyngeal carcinoma (NPC), as a model and developed a powerful assay based on ‘‘multiplex methylation specific-PCR (MMSP)’’. The MMSP assay was designed to detect tumor-specific methylation status of several NPC-related genes and was capable of acquiring multiplex information simultaneously through a single PCR reaction with the tiny tumor DNA derived from the direct body fluid close to the primary tumor. In this study, we collected paired