defining global neuroendocrine gene expression patterns associated with reproductive seasonality in fish定义全球神经内分泌与鱼的繁殖季节性相关基因表达模式.pdfVIP

Defining Global Neuroendocrine Gene Expression Patterns Associated with Reproductive Seasonality in Fish 1. 1. 1 1 1 Dapeng Zhang , Huiling Xiong , Jan A. Mennigen , Jason T. Popesku , Vicki L. Marlatt , Christopher J. 1 1 2 1 1 Martyniuk , Kate Crump , Andrew R. Cossins , Xuhua Xia , Vance L. Trudeau * 1 Centre for Advanced Research in Environmental Genomics (CAREG), Department of Biology, University of Ottawa, Ottawa, Ontario, Canada, 2 School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom Abstract Background: Many vertebrates, including the goldfish, exhibit seasonal reproductive rhythms, which are a result of interactions between external environmental stimuli and internal endocrine systems in the hypothalamo-pituitary-gonadal axis. While it is long believed that differential expression of neuroendocrine genes contributes to establishing seasonal reproductive rhythms, no systems-level investigation has yet been conducted. Methodology/Principal Findings: In the present study, by analyzing multiple female goldfish brain microarray datasets, we have characterized global gene expression patterns for a seasonal cycle. A core set of genes (873 genes) in the hypothalamus were identified to be differentially expressed between May, August and December, which correspond to physiologically distinct stages that are sexually mature (prespawning), sexual regression, and early gonadal redevelopment, respectively. Expression changes of these genes are also shared by another brain region, the telenceph