defining global gene expression changes of the hypothalamic-pituitary-gonadal axis in female sgnrh-antisense transgenic common carp (cyprinus carpio)定义全球基因表达变化的hypothalamic-pituitary-gonadal轴在女性sgnrh-antisense转基因鲤鱼(鲤属carpio).pdfVIP

Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH- Antisense Transgenic Common Carp (Cyprinus carpio) 1,2 1 1 1 1 1 1 Jing Xu , Wei Huang , Chengrong Zhong , Daji Luo , Shuangfei Li , Zuoyan Zhu , Wei Hu * 1 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China, 2 Graduate School of the Chinese Academy of Sciences, Beijing, China Abstract Background: The hypothalamic-pituitary-gonadal (HPG) axis is critical in the development and regulation of reproduction in fish. The inhibition of neuropeptide gonadotropin-releasing hormone (GnRH) expression may diminish or severely hamper gonadal development due to it being the key regulator of the axis, and then provide a model for the comprehensive study of the expression patterns of genes with respect to the fish reproductive system. Methodology/Principal Findings: In a previous study we injected 342 fertilized eggs from the common carp (Cyprinus carpio) with a gene construct that expressed antisense sGnRH. Four years later, we found a total of 38 transgenic fish with abnormal or missing gonads. From this group we selected the 12 sterile females with abnormal ovaries in which we combined suppression subtractive hybridization (SSH) and cDNA microarray analysis to define changes in gene expression of the HPG axis in the present study. As a result, nine, 28, and 212 genes were separately identified as being differentially expressed in hypothalamus, pituitary, and ovary, of which 87 genes were novel. The number of down- and up-regulated genes was five and four (hypothalamus), 16 and 12 (pituitary), 119 and 93 (ovary), respectively. Functio