drosophila cuticular hydrocarbons revisited mating status alters cuticular profiles果蝇交配表皮碳氢化合物重新审视状态改变表皮概要文件.pdfVIP

Drosophila Cuticular Hydrocarbons Revisited: Mating Status Alters Cuticular Profiles 1 . 1. 2 1 Claude Everaerts * , Jean-Pierre Farine , Matthew Cobb , Jean-Franc¸ois Ferveur ˆ ´ 1 Centre des Sciences du Gout et de l’Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Universite de Bourgogne, Agrosup, Dijon, France, 2 Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom Abstract Most living organisms use pheromones for inter-individual communication. In Drosophila melanogaster flies, several pheromones perceived either by contact/at a short distance (cuticular hydrocarbons, CHs), or at a longer distance (cis- vaccenyl acetate, cVA), affect courtship and mating behaviours. However, it has not previously been possible to precisely identify all potential pheromonal compounds and simultaneously monitor their variation on a time scale. To overcome this limitation, we combined Solid Phase Micro-Extraction with gas-chromatography coupled with mass-spectrometry. This allowed us (i) to identify 59 cuticular compounds, including 17 new CHs; (ii) to precisely quantify the amount of each compound that could be detected by another fly, and (iii) to measure the variation of these substances as a function of aging and mating. Sex-specific variation appeared with age, while mating affected cuticular compounds in both sexes with three possible patterns: variation was (i) reciprocal in the two sexes, suggesting a passive mechanical transfer during mating, (ii) parallel in both sexes, such as for cVA which strikingly appeared during