effect of b7.1 costimulation on t-cell based immunity against tap-negative cancer can be facilitated by tap1 expressionb7.1聚集有关对基于t细胞免疫的影响对tap-negative癌症可以促进tap1表达式.pdfVIP

Effect of B7.1 Costimulation on T-Cell Based Immunity against TAP-Negative Cancer Can Be Facilitated by TAP1 Expression 1 3 1 1 1 2 Xiao-Lin Li , Yong-Yu Liu , David Knight , Yoshinobu Odaka , J. Michael Mathis , Runhua Shi , Jonathan 2 1 Glass , Qian-Jin Zhang * 1 Department of Cellular Biology and Anatomy, Gene Therapy Program, Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States of America, 2 Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States of America, 3 College of Pharmacy, Basic Pharmaceutical Sciences, University of Louisiana, Monroe, Louisiana, United States of America Abstract Tumors deficient in expression of the transporter associated with antigen processing (TAP) usually fail to induce T-cell- mediated immunity and are resistant to T-cell lysis. However, we have found that introduction of the B7.1 gene into TAP- negative (TAP2 + ) or TAP1-transfected (TAP1 ) murine lung carcinoma CMT.64 cells can augment the capacity of the cells to induce a protective immune response against wild-type tumor cells. Differences in the strength of the protective immune responses were observed between TAP2 and TAP1+ B7.1 expressing CMT.64 cells depending on the doses of c-irradiated cell immunization. While mice immunized with either high or low dose of B7.1-expressing TAP1+ cells rejected TAP2 tumors, only high dose immunization with B7.1-expressing TAP2 cells resulted in tumor rejection. The induced protective immunity was T-cell dependent as demonstrated by dramatically reduced antitumor im