drosophila hp1c is regulated by an auto-regulatory feedback loop through its binding partner woc果蝇hp1c受是一个通过其约束力的合作伙伴woc auto-regulatory反馈回路.pdfVIP

Drosophila HP1c Is Regulated by an Auto-Regulatory Feedback Loop through Its Binding Partner Woc 1 1¤ 2 3 1 Jochen Abel , Ragnhild Eskeland , Grazia D. Raffa , Elisabeth Kremmer , Axel Imhof * 1 Munich Centre of Integrated Protein Science and Adolf-Butenandt Institute, Ludwig Maximilians University of Munich, Munich, Germany, 2 Dipartimento di Genetica e ¨ Biologia Molecolare Universita’ di Roma La Sapienza, Piazzale Aldo Moro, Rome, Italy, 3 Helmholtz Zentrum Munchen, Institute of Molecular Immunology, Munich, Germany Abstract HP1 is a major component of chromatin and regulates gene expression through its binding to methylated histone H3. Most eukaryotes express at least three isoforms of HP1 with similar domain architecture. However, despite the common specificity for methylated histone H3, the three HP1 isoforms bind to different regions of the genome. Most of the studies so far focused on the HP1a isoform and its role in transcriptional regulation. As HP1a requires additional factors to bind methylated chromatin in vitro, we wondered whether another isoform might also require additional targeting factors. Indeed, we found that HP1c interacts with the DNA binding factors Woc and Row and requires Woc to become targeted to chromatin in vivo. Moreover, we show that the interaction between HP1c and Woc constitutes a transcriptional feedback loop that operates to balance the concentration of HP1c within the cell. This regulation may prevent HP1c from binding to methylated heterochromatin. Citation: Abel J, Eskeland R, Raffa GD, Kremmer E, Imhof