distinct origin of the y and st genome in elymus species evidence from the analysis of a large sample of st genome species using two nuclear genes不同y和圣大物种基因组起源证据的分析大样本的圣基因组物种使用两个核基因.pdfVIP

Distinct Origin of the Y and St Genome in Elymus Species: Evidence from the Analysis of a Large Sample of St Genome Species Using Two Nuclear Genes 1,2 1,2 1 Chi Yan , Genlou Sun *, Dongfa Sun * 1 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China, 2 Biology Department, Saint Mary’s University, Halifax, Nova Scotia, Canada Abstract Background: Previous cytological and single copy nuclear genes data suggested the St and Y genome in the StY-genomic Elymus species originated from different donors: the St from a diploid species in Pseudoroegneria and the Y from an unknown diploid species, which are now extinct or undiscovered. However, ITS data suggested that the Y and St genome shared the same progenitor although rather few St genome species were studied. In a recent analysis of many samples of St ` ¨ genome species Pseudoroegneria spicata (Pursh) A. Love suggested that one accession of P. spicata species was the most likely donor of the Y genome. The present study tested whether intraspecific variation during sampling could affect the outcome of analyses to determining the origin of Y genome in allotetraploid StY species. We also explored the evolutionary dynamics of these species. Methodology/Principal Findings: Two single copy nuclear genes, the second largest subunit of RNA polymerase II (RPB2) and the translation elongation factor G (EF-G) sequences from 58 accessions of Pseudoroegneria and Elymus species, together with those from Hordeum (H), Agropyron (P), Australopyrum (W), Lophopyrum (Ee a