differentially expressed rna from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions差异表达rna从公共微阵列数据确定血清蛋白生物标记对cross-organ移植排斥和其他条件.pdfVIP

Differentially Expressed RNA from Public Microarray Data Identifies Serum Protein Biomarkers for Cross- Organ Transplant Rejection and Other Conditions 1,2 1,2 1,2 3 1,2 1,2 Rong Chen , Tara K. Sigdel , Li Li , Neeraja Kambham , Joel T. Dudley , Szu-chuan Hsieh , 1,2 1,2 4 1,2 4 R. Bryan Klassen , Amery Chen , Tuyen Caohuu , Alexander A. Morgan , Hannah A. Valantine , Kiran K. Khush4, Minnie M. Sarwal 1,2*, Atul J. Butte1,2* 1 Department of Pediatrics, Stanford University School of Medicine, Stanford, California, United States of America, 2 Lucile Packard Children’s Hospital, Palo Alto, California, United States of America, 3 Department of Pathology, Stanford University School of Medicine, Stanford, California, United States of America, 4 Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America Abstract Serum proteins are routinely used to diagnose diseases, but are hard to find due to low sensitivity in screening the serum proteome. Public repositories of microarray data, such as the Gene Expression Omnibus (GEO), contain RNA expression profiles for more than 16,000 biological conditions, covering more than 30% of United States mortality. We hypothesized that genes coding for serum- and urine-detectable proteins, and showing differential expression of RNA in disease-damaged tissues would make ideal diagnostic protein biomarkers for those diseases. We showed that predicted protein biomarkers are significantly enriched f