development of a quantitative bead capture assay for soluble il-7 receptor alpha in human plasma发展定量珠捕捉试验在人血浆可溶性il-7受体α.pdfVIP

Development of a Quantitative Bead Capture Assay for Soluble IL-7 Receptor Alpha in Human Plasma 1 2,3 1 1 1 Sylvie Faucher *, Angela M. Crawley , Wendy Decker , Alice Sherring , Dragica Bogdanovic , Tao 1 1 2,3,4 1 Ding , Michele Bergeron , Jonathan B. Angel , Paul Sandstrom 1 National HIV and Retrovirology Laboratories, Public Health Agency of Canada, Ottawa, Canada, 2 Ottawa Hospital Research Institute, Ottawa, Canada, 3 Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada, 4 Division of Infectious Diseases, Ottawa Hospital-General Campus, Ottawa, Canada Abstract Background: IL-7 is an essential cytokine in T-cell development and homeostasis. It binds to the IL-7R receptor, a complex of the IL-7Ra (CD127) and common c (CD132) chains. There is significant interest in evaluating the expression of CD127 on human T-cells as it often decreased in medical conditions leading to lymphopenia. Previous reports showed the usefulness of CD127 as a prognostic marker in viral infections such as HIV, CMV, EBV and HCV. A soluble CD127 (sCD127) is released in plasma and may contribute to disease pathogenesis through its control on IL-7 activities. Measuring sCD127 is important to define its role and may complement existing markers used in lymphopenic disease management. We describe a new quantitative assay for the measurement of sCD127 in plasma and report sCD127 concentrations in healthy adults. Methodology/Principal Findings: We developed a quantitative bead-based sCD127 capture assay. Polyclonal CD127- specific antibodies were chosen for capture and a bio