defining the molecular character of the developing and adult kidney podocyte定义的分子特征的发展和成人肾脏足细胞.pdfVIP

Defining the Molecular Character of the Developing and Adult Kidney Podocyte 1 2 2 2 1 Eric W. Brunskill , Kylie Georgas , Bree Rumballe , Melissa H. Little , S. Steven Potter * 1 Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center and the University of Cincinnati School of Medicine, Cincinnati, Ohio, United States of America, 2 Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Australia Abstract Background: The podocyte is a remarkable cell type, which encases the capillaries of the kidney glomerulus. Although mesodermal in origin it sends out axonal like projections that wrap around the capillaries. These extend yet finer projections, the foot processes, which interdigitate, leaving between them the slit diaphragms, through which the glomerular filtrate must pass. The podocytes are a subject of keen interest because of their key roles in kidney development and disease. Methodology/Principal Findings: In this report we identified and characterized a novel transgenic mouse line, MafB-GFP, which specifically marked the kidney podocytes from a very early stage of development. These mice were then used to facilitate the fluorescent activated cell sorting based purification of podocytes from embryos at E13.5 and E15.5, as well as adults. Microarrays were then used to globally define the gene expression states of podocytes at these different developmental stages. A remarkable picture emerged, identifying the multiple sets of genes that establish the neuronal, muscle, and phagocytic properties of podocytes. The complete combinatorial code of transcription factors that create th