differential susceptibility of interneurons expressing neuropeptide y or parvalbumin in the aged hippocampus to acute seizure activity微分磁化率的中间神经元表达神经肽y或小清蛋白在老年人海马急性发作.pdfVIP

Differential Susceptibility of Interneurons Expressing Neuropeptide Y or Parvalbumin in the Aged Hippocampus to Acute Seizure Activity Ramkumar Kuruba1,4, Bharathi Hattiangady1,2,4, Vipan K. Parihar1,4, Bing Shuai1,2,4, Ashok K. Shetty1,2,3,4* 1 Medical Research and Surgery Services, Veterans Affairs Medical Center, Durham, North Carolina, United States of America, 2 Institute for Regenerative Medicine, Texas AM Health Science Center at Scott White, Temple, Texas, United States of America, 3 Research Service, Central Texas Veterans Health Care System, Temple, Texas, United States of America, 4 Division of Neurosurgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America Abstract Acute seizure (AS) activity in old age has an increased predisposition for evolving into temporal lobe epilepsy (TLE). Furthermore, spontaneous seizures and cognitive dysfunction after AS activity are often intense in the aged population than in young adults. This could be due to an increased vulnerability of inhibitory interneurons in the aged hippocampus to AS activity. We investigated this issue by comparing the survival of hippocampal GABA-ergic interneurons that contain the neuropeptide Y (NPY) or the calcium binding protein parvalbumin (PV) between young adult (5-months old) and aged (22- months old) F344 rats at 12 days after three-hours of AS activity. Graded intraperitoneal injections of the kainic acid (KA) induced AS activity and a diazepam injection at 3 hours after the onset terminated AS-activity. Measurement of interneuron numbers in different hippocampal subfields revealed that NPY+ interneurons were relatively resistant to AS activity in the aged hippocampus in comparison to the young adult hippocampus. Whereas, PV+ interneurons were highly susceptible to AS activity in both age groups. However, as aging alone substantial