differential use of signal peptides and membrane domains is a common occurrence in the protein output of transcriptional units微分信号肽和膜的使用域是一种常见的发生在蛋白质转录单位的输出.pdfVIP

Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units 1 1 1,2 1 1 3 3 Melissa J. Davis , Kelly A. Hanson , Francis Clark , J. Lynn Fink , Fasheng Zhang , Takeya Kasukawa , Chikatoshi Kai , Jun Kawai3,4, Piero Carninci3,4, Yoshihide Hayashizaki3,4, Rohan D. Teasdale1* 1 Institute for Molecular Bioscience and ARC Centre in Bioinformatics, University of Queensland, St. Lucia, Queensland, Australia, 2 Advanced Computational Modeling Centre, University of Queensland, St. Lucia, Queensland, Australia, 3 Genome Exploration Research Group (Genome Network Project Core Group), RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama, Japan, 4 Genome Science Laboratory, Discovery Research Institute, RIKEN Wako Institute, Wako, Japan Membrane organization describes the orientation of a protein with respect to the membrane and can be determined by the presence, or absence, and organization within the protein sequence of two features: endoplasmic reticulum signal peptides and alpha-helical transmembrane domains. These features allow protein sequences to be classified into one of five membrane organization categories: soluble intracellular proteins, soluble secreted proteins, type I membrane proteins, type II membrane proteins, and multi-spanning membrane proteins. Generation of protein isoforms with variable membrane organizations can change a protein’s subcellular localization or association with the membrane. Application of MemO, a membrane organization annotation pipeline, to the FANTOM3 Isoform Protein Sequence mouse protein set revealed that within the 8,032 transcriptional units (TUs) with multiple protein i