distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage不同种群的肝星状细胞在小鼠肝脏有不同的能力类维生素a和脂质存储.pdfVIP

Distinct Populations of Hepatic Stellate Cells in the Mouse Liver Have Different Capacities for Retinoid and Lipid Storage 1,2 ´ 1 1 1 3 Diana N. D’Ambrosio , Jose L. Walewski , Robin D. Clugston , Paul D. Berk , Richard A. Rippe , William S. Blaner1,2* 1 Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America, 2 Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, New York, New York, United States of America, 3 Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America Abstract Hepatic stellate cell (HSC) lipid droplets are specialized organelles for the storage of retinoid, accounting for 50–60% of all retinoid present in the body. When HSCs activate, retinyl ester levels progressively decrease and the lipid droplets are lost. The objective of this study was to determine if the HSC population in a healthy, uninjured liver demonstrates heterogeneity in its capacity for retinoid and lipid storage in lipid droplets. To this end, we utilized two methods of HSC isolation, which leverage distinct properties of these cells, including their vitamin A content and collagen expression. HSCs were isolated either from wild type (WT) mice in the C57BL/6 genetic background by flotation in a Nycodenz density gradient, followed by fluorescence activated cell sorting (FACS) based on vitamin A autofluorescence, or from collagen-green fluorescent protein (GFP) mice by FACS based on GFP expression from a GFP transgene driven by the collagen I promoter. We show that GFP- HSCs have: (i) increased expression of typical markers of HS