differential seroprevalence of human bocavirus species 1-4 in beijing, china微分seroprevalence人类bocavirus物种1 - 4在北京,中国.pdfVIP

Differential Seroprevalence of Human Bocavirus Species 1-4 in Beijing, China 1. 1. 1 1 2 1 ´ Li Guo , Yaying Wang , Hongli Zhou , Chao Wu , Jingdong Song , Jianguo Li , Glaucia Paranhos- ` 3 3 1 1,2 Baccala , Guy Vernet , Jianwei Wang *, Tao Hung ´ ´ 1 MOH Key Laboratory of Systems Biology of Pathogens and Christophe Merieux Laboratory, IPB, CAMS-Fondation Merieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) Peking Union Medical College (PUMC), Beijing, People’s Republic of China, 2 National Institute for Viral Disease Control and ´ Prevention, Chinese Center for Disease control and Prevention, Beijing, People’s Republic of China, 3 Fondation Merieux, Lyon, France Abstract Background: Four species of human bocaviruses (HBoV1-4) have been identified based on phylogenetic analysis since its first report in 2005. HBoV1 has been associated with respiratory disease, whereas HBoV2-4 are mainly detected in enteric infections. Although the prevalence of HBoVs in humans has been studied in some regions, it has not been well addressed globally. Methodology/Principal Findings: Cross-reactivity of anti-VP2 antibodies was detected between HBoV1, 2, 3, and 4 in mouse and human serum. The prevalence of specific anti-VP2 IgG antibodies against HBoV1-4 was determined in different age groups of healthy individuals aged 0-70 years old in Beijing, China, using a competition ELISA assay based on virus-like p