differentiation of human embryonic stem cells to regional specific neural precursors in chemically defined medium conditions人类胚胎干细胞的分化区域特定神经前体的化学介质条件下定义的.pdfVIP

Differentiation of Human Embryonic Stem Cells to Regional Specific Neural Precursors in Chemically Defined Medium Conditions 1 ´ 2 1 1 ´ ´ 1 Slaven Erceg , Sergio Laınez , Mohammad Ronaghi , Petra Stojkovic , Maria Amparo Perez-Arago , 1 ´ 1 2 1 Victoria Moreno-Manzano , Ruben Moreno-Palanques , Rosa Planells-Cases , Miodrag Stojkovic * ´ ´ ´ ´ 1 Cellular Reprogramming Laboratory, Centro de Investigacion Prıncipe Felipe (CIPF), Valencia, Spain, 2 Sensorial Biology Laboratory, Centro de Investigacion Prıncipe Felipe (CIPF), Valencia, Spain Abstract Background: Human embryonic stem cells (hESC) provide a unique model to study early events in human development. The hESC-derived cells can potentially be used to replace or restore different tissues including neuronal that have been damaged by disease or injury. Methodology and Principal Findings: The cells of two different hESC lines were converted to neural rosettes using adherent and chemically defined conditions. The progenitor cells were exposed to retinoic acid (RA) or to human recombinant basic fibroblast growth factor (bFGF) in the late phase of the rosette formation. Exposing the progenitor cells to RA suppressed differentiation to rostral forebrain dopamine neural lineage and promoted that of spinal neural tissue includi