development of a surface plasmon resonance biosensor for real-time detection of osteogenic differentiation in live mesenchymal stem cells表面等离子体共振生物传感器实时检测的发展生活间充质干细胞的成骨分化.pdfVIP

Development of a Surface Plasmon Resonance Biosensor for Real-Time Detection of Osteogenic Differentiation in Live Mesenchymal Stem Cells 1,2 3,4,5 6 7 1,2,7,8 Yi-Chun Kuo , Jennifer H. Ho , Ta-Jen Yen , How-Foo Chen *, Oscar Kuang-Sheng Lee * 1 Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, 2 Stem Cell Research Center, National Yang-Ming University, Taipei, Taiwan, 3 Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei, Taiwan, 4 Center for Stem Cell Research, Taipei Medical University-Wan Fang Medical Center, Taipei, Taiwan, 5 Department of Ophthalmology, Taipei Medical University-Wan Fang Medical Center, Taipei, Taiwan, 6 Department of Material Science and Engineering, National Tsing Hua University, Hsinchu, Taiwan, 7 Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, 8 Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan Abstract Surface plasmon resonance (SPR) biosensors have been recognized as a useful tool and widely used for real-time dynamic analysis of molecular binding affinity because of its high sensitivity to the change of the refractive index of tested objects. The conventional methods in molecular biology to evaluate cell differentiation require cell lysis or fixation, which make investigation in live cells difficult. In addition, a certain amount of cells are needed in order to obtain adequate protein or messenger ribonucleic acid for various assays. To overcome this limitation, we developed a unique SPR-based biosensing apparatus for real-time detection of cell differentiation in live cells according to the differences of optical properties of