development of a novel fluorophore for real-time biomonitoring system为实时生物监测系统发展的一种新型的荧光团.pdfVIP

Development of a Novel Fluorophore for Real-Time Biomonitoring System 1 1 2 3 3 4 Hyun-Ok Song , Binna Lee , Ram Prasad Bhusal , Byounghun Park , Kyoungsik Yu , Chom-Kyu Chong , 1¤ 2 2 1 PyoYun Cho , Sung Yeon Kim , Hak Sung Kim *, Hyun Park * 1 Zoonosis Research Center, Department of Infection Biology, Wonkwang University College of Medicine, Iksan, Jeonbuk, Republic of Korea, 2 College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University, Iksan, Jeonbuk, Republic of Korea, 3 Department of Electrical Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea, 4 Department of Biochemistry, Division of Life Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea Abstract Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic results to a control center in order to systematically prevent disease outbreaks. In this study, we developed a novel fluorophore, coumarin-derived dendrimer, with high emission intensity, strong signal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590 nm with excitation at 455 nm. Application to fluorescent immunochromatogr