development of functional genomic tools in trematodes rna interference and luciferase reporter gene activity in fasciola hepatica吸虫类rna干扰功能基因组工具的发展和肝片吸虫荧光素酶报告基因活性.pdfVIP

Development of Functional Genomic Tools in Trematodes: RNA Interference and Luciferase Reporter Gene Activity in Fasciola hepatica 1,2 1 ´ 2 3 1¤ ´ 2 Gabriel Rinaldi , Maria E. Morales , Martın Cancela , Estela Castillo , Paul J. Brindley , Jose F. Tort * ´ 1 Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, Louisiana, United States of America, 2 Departamento de Genetica, Facultad de ´ ´ ´ ´ Medicina, Universidad de la Republica, Udelar, Montevideo, Uruguay, 3 Seccion Bioquımica, Facultad de Ciencias, Universidad de la Republica, Udelar, Montevideo, Uruguay Abstract The growing availability of sequence information from diverse parasites through genomic and transcriptomic projects offer new opportunities for the identification of key mediators in the parasite–host interaction. Functional genomics approaches and methods for the manipulation of genes are essential tools for deciphering the roles of genes and to identify new intervention targets in parasites. Exciting advances in functional genomics for parasitic helminths are starting to occur, with transgene expression and RNA interference (RNAi) reported in several species of nematodes, but the area is still in its infancy in flatworms, with reports in just three species. While advancing in model organisms, there is a need to rapidly extend these technologies to other parasite