differentiation of glioma and radiation injury in rats using in vitro produce magnetically labeled cytotoxic t-cells and mri分化的神经胶质瘤和辐射损伤大鼠使用体外生产磁性标记细胞毒性t细胞和核磁共振.pdfVIP

Differentiation of Glioma and Radiation Injury in Rats Using In Vitro Produce Magnetically Labeled Cytotoxic T-Cells and MRI 1 1 1 1 3 Ali S. Arbab *, Branislava Janic , Kourosh Jafari-Khouzani , A. S. M. Iskander , Sanath Kumar , 1 2 1 3 Nadimpalli R. S. Varma , Robert A. Knight , Hamid Soltanian-Zadeh , Stephen L. Brown , Joseph A. Frank4,5 1 Cellular and Molecular Imaging Laboratory, Department of Radiology, Henry Ford Hospital, Detroit, Michigan, United States of America, 2 Department of Neurology, Henry Ford Hospital, Detroit, Michigan, United States of America, 3 Department of Radiation Oncology, Henry Ford Hospital, Detroit, Michigan, United States of America, 4 Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, United States of America, 5 Frank Laboratory, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, United States of America Abstract Background: A limitation with current imaging strategies of recurrent glioma undergoing radiotherapy is that tumor and radiation injury cannot be differentiated with post contrast CT or MRI, or with PET or other more complex parametric analyses of MRI data. We propose to address the imaging limitation building on emerging evidence indicating that effective therapy for recurrent glioma can be attained by sensitized T-cells following vaccination of primed dendritic cells (DCs). The purpose of this study was to determine whether cord blood T-cells can be sensitized against glioma cells (U-251) and if these sensitized cytotoxic T-cells (C