differentiation and recruitment of th9 cells stimulated by pleural mesothelial cells in human mycobacterium tuberculosis infection分化和招聘th9细胞刺激胸膜间皮的细胞在人类结核分枝杆菌感染.pdfVIP

Differentiation and Recruitment of Th9 Cells Stimulated by Pleural Mesothelial Cells in Human Mycobacterium tuberculosis Infection 1. 1. 1 2 1 1 Zhi-Jian Ye , Ming-Li Yuan , Qiong Zhou , Rong-Hui Du , Wei-Bing Yang , Xian-Zhi Xiong , 1 3 3 1 Jian-Chu Zhang , Cong Wu , Shou-Ming Qin , Huan-Zhong Shi * 1 Key Laboratory of Pulmonary Diseases of Health Ministry, Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, 2 Department of Internal Medicine, Wuhan Institute of Tuberculosis Prevention and Control, Wuhan, China, 3 Institute of Respiratory Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, China Abstract Newly discovered IL-9–producing CD4+ helper T cells (Th9 cells) have been reported to contribute to tissue inflammation and immune responses, however, differentiation and immune regulation of Th9 cells in tuberculosis remain unknown. In the present study, our data showed that increased Th9 cells with the phenotype of effector memory cells were found to be in ¨ + tuberculous pleural effusion as compared with blood. TGF- b was essential for Th9 cell differentiation from naıve CD4 T cells stimulated with PMA and ionomycin in vitro for 5 h, and addition of IL-1 b, IL-4 or IL-6 further augmented Th9 cell differentiation. Tuberculous pleural effusion and supernatants of cultured