differentiating protein-coding and noncoding rna challenges and ambiguities区分蛋白质编码和非编码rna的挑战和含糊之处.pdfVIP

Review Differentiating Protein-Coding and Noncoding RNA: Challenges and Ambiguities 1. 2. 1. 1 Marcel E. Dinger , Ken C. Pang , Tim R. Mercer , John S. Mattick * 1 ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia, 2 T cell Laboratory, Ludwig Institute for Cancer Research, Melbourne Centre for Clinical Sciences, Austin Health, Heidelberg, Australia categories (e.g., microRNAs, PIWI-associated RNAs, and endog- Abstract: The assumption that RNA can be readily enous small interfering RNAs) on the basis of function, length, classified into either protein-coding or non-protein– biogenesis, structural/sequence features, and protein-binding coding categories has pervaded biology for close to 50 partners (reviewed in [12]). Interestingly, however, long ncRNAs years. Until recently, discrimination between these two (.200 nt) appear to comprise the largest portion of the categories was relatively straightforward: most transcripts mammalian noncoding transcriptome. Tiling array studies of the were clearly identifiable as protein-coding messenger human genome, for instance, revealed that the majority of RNAs (mRNAs), and readily distinguished from the small number of well-characterized non-protein–coding RNAs transcription occurs as long ncR