drhogef2 regulates cellular tension and cell pulsations in the amnioserosa during drosophila dorsal closuredrhogef2调节细胞张力和细胞脉动在amnioserosa果蝇背封.pdfVIP

DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in the Amnioserosa during Drosophila Dorsal Closure 1 1 1 2 3 4 Dulce Azevedo , Marco Antunes , Soren Prag , Xiaoyan Ma , Udo Hacker , G. Wayne Brodland , M. 2 5 1,6 Shane Hutson , Jerome Solon , Antonio Jacinto * 1 Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal, 2 Vanderbilt Institute for Integrative Biosystems Research and Education, Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee, United States of America, 3 Department of Experimental Medical Science, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden, 4 Department of Civil and Environmental Engineering, University of ˆ Waterloo, Waterloo, Ontario, Canada, 5 CRG - Centre for Genomic Regulation, Barcelona, Spain, 6 Instituto Gulbenkian de Ciencia, Oeiras, Portugal Abstract Coordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in the contraction time of pulsations. We probed the physical properties of the amnioserosa to show that the average tension in DRhoGEF2 mutant cells is lower than wild-type and that overexpression of