distinct roles of comk1 and comk2 in gene regulation in bacillus cereus不同的角色comk1和comk2在蜡样芽胞杆菌的基因调控.pdfVIP

Distinct Roles of ComK1 and ComK2 in Gene Regulation in Bacillus cereus ´ 1 ˇ 1 1,2 ´ ´ 1 Aleksandra M. Mironczuk , Amagoia Manu , Oscar P. Kuipers *, Akos T. Kovacs 1 Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands, 2 Kluyver Centre for Genomics of Industrial Fermentation, Groningen, The Netherlands Abstract The B. subtilis transcriptional factor ComK regulates a set of genes coding for DNA uptake from the environment and for its integration into the genome. In previous work we showed that Bacillus cereus expressing the B. subtilis ComK protein is able to take up DNA and integrate it into its own genome. To extend our knowledge on the effect of B. subtilis ComK overexpression in B. cereus we first determined which genes are significantly altered. Transcriptome analysis showed that only part of the competence gene cluster is significantly upregulated. Two ComK homologues can be identified in B. cereus that differ in their respective homologies to other ComK proteins. ComK1 is most similar, while ComK2 lacks the C-terminal region previously shown to be important for transcription activation by B. subtilis ComK. comK1 and comK2 overexpression and deletion studies using transcriptomics techniques showed that ComK1 enhances and ComK2 decreases expression of the comG operon, when B. subtilis ComK was overexpressed simultaneously. ´ ´ ˇ ´ Citation: Mironczuk AM, Manu A, Kuipers OP, Kovacs AT (2011) Distinct Roles of ComK1 and ComK2