distinct roles of bcl-2 and bcl-xl in the apoptosis of human bone marrow mesenchymal stem cells during differentiation不同角色的bcl - 2和bcl-xl人类骨髓间充质干细胞的细胞凋亡在分化.pdfVIP

Distinct Roles of Bcl-2 and Bcl-Xl in the Apoptosis of Human Bone Marrow Mesenchymal Stem Cells during Differentiation 1,2 1,2 2,3 1,2 4 Lisa Oliver , Erika Hue , Julien Rossignol , Gwenola Bougras , Philippe Hulin , Philippe Naveilhan2,3, Dominique Heymann2,5, Laurent Lescaudron2,3,6, Franc¸ois M. Vallette1,2* ´ ´ ´ ´ ´ 1 INSERM, UMR 892, Equipe Labellisee «Ligue contre le Cancer», Nantes, France, 2 Faculte de Medecine, Universite de Nantes, Nantes, France, 3 INSERM, UMR 643, Nantes, ´ France, 4 Cellular and Tissular Imaging Core Facility (MicroPICell), Universite de Nantes, Nantes, France, 5 INSERM, UMR 957, Nantes, France, 6 Service de Physiologie ´ Animale et Humaine, UFR Sciences et Techniques, Universite de Nantes, Nantes, France Abstract Background: Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells. Principal Findings: Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in response to different cell death inducers. Conversely, the same inducers can readily induce apoptosis when hMSCs are engaged in the early stages of differentiation. The survival of undifferentiated cells is linked to the expression of Bcl-Xl and Bcl-2 in completely opposite ways. Bcl-Xl is expressed at similar levels in undifferentiated and differentiated hMSCs while Bcl-2 is expr