fis is essential for the stability of linear plasmid pbssb1 and affects the motility of salmonella enterica serovar typhifis对线性稳定的质粒pbssb1至关重要,影响伤寒沙门氏菌血清型的能动性.pdfVIP

下载本文档

4
0
约3.85万字
约 5页
2017-09-01 发布于上海
举报
版权申诉

fis is essential for the stability of linear plasmid pbssb1 and affects the motility of salmonella enterica serovar typhifis对线性稳定的质粒pbssb1至关重要,影响伤寒沙门氏菌血清型的能动性.pdf

1、本文档共5页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

fis is essential for the stability of linear plasmid pbssb1 and affects the motility of salmonella enterica serovar typhifis对线性稳定的质粒pbssb1至关重要,影响伤寒沙门氏菌血清型的能动性

Fis Is Essential for the Stability of Linear Plasmid pBSSB1 and Affects the Motility of Salmonella enterica Serovar Typhi 1 1 1 1 1 2 1 1 Haifang Zhang , Bin Ni , Xin Zhao , Isaac Dadzie , Hong Du , Qiang Wang , Huaxi Xu , Xinxiang Huang * 1 Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu, China, 2 State Key Laboratory of Pharmaceutical Biotechnology, Department of Biology, Nanjing University, Nanjing, Jiangsu, China Abstract pBSSB1 is a 27 kb non-bacteriophage-related linear plasmid first found in Salmonella enterica serovar Typhi (S. Typhi), but the mechanism underlying the replication of pBSSB1 is currently unknown. Previous reports showed that the factor for inversion stimulation (Fis) encoded by fis can affect the replication, transcription and other processes through binding DNA. Here, a fis deletion mutant of S. Typhi (Dfis) was prepared through the homologous recombination mediated by suicide plasmid and the loss of pBSSB1 in Dfis was observed surprisingly by pulsed field gel electrophoresis (PFGE). Subsequently, the loss of pBSSB1 was verified by PCR and Southern blot. In addition, the motility of Dfis was deficient and the flagellin of Dfis could not be detected by 2-dimensional polyacrylamide gel electrophoresis. All these results show that Fis is essential for the stability of pBSSB1 and affects the motility of S. Typhi. Citation: Zhang H, Ni B, Zhao X, Dadzie I, Du H, et al. (2012) Fis Is Essential for the Stability of Linear Plasmid pBSSB1 and Affects the Motility of Salmonella enterica Serovar Typhi. PLoS ONE 7(7): e37462. doi:10.1371/journal.pone.0037462 Editor: Michael Hensel, University of Osnabrueck, Germany Received Decem