functional dissection of hoxd cluster genes in regulation of neuroblastoma cell proliferation and differentiation集群的功能解剖hoxd基因在神经母细胞瘤细胞增殖和分化的调控.pdfVIP

Functional Dissection of HOXD Cluster Genes in Regulation of Neuroblastoma Cell Proliferation and Differentiation 1,4. 3. 1 1,5 1,6 1 Yunhong Zha , Emily Ding , Liqun Yang , Ling Mao , Xiangwei Wang , Brian A. McCarthy , 2 1 Shuang Huang , Han-Fei Ding * 1 Cancer Center and Department of Pathology, Medical College of Georgia, Georgia Health Sciences University, Augusta, Georgia, United States of America, 2 Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Health Sciences University, Augusta, Georgia, United States of America, 3 Lakeside High School, Evans, Georgia, United States of America, 4 Department of Neurology, the First People’s Hospital of Yichang, Three Gorges University College of Medicine, Yichang, Hubei, China, 5 Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China, 6 Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China Abstract Retinoic acid (RA) can induce growth arrest and neuronal differentiation of neuroblastoma cells and has been used in clinic for treatment of neuroblastoma. It has been reported that RA induces the expression of several HOXD genes in human neuroblastoma cell lines, but their roles in RA action are largely unknown. The HOXD cluster contains nine genes (HOXD1, HOXD3, HOXD4, and HOXD8-13) that are positioned sequentially from 39 to 59, with HOXD1 at the 39 end and HOXD13 the 59 end. Here we show that all HOXD genes are induced by RA in the human neuroblastoma BE(2)-C cells, with the genes located at the 39 end being activate