genomic signature and mutation trend analysis of pandemic (h1n1) 2009 influenza a virus基因组特征和变异趋势分析的2009年h1n1流感大流行流感病毒.pdfVIP

Genomic Signature and Mutation Trend Analysis of Pandemic (H1N1) 2009 Influenza A Virus 1,2 1 1,3 4 1,3 3 1,3 Chungen Pan , Byron Cheung , Suiyi Tan , Chunling Li , Lin Li , Shuwen Liu , Shibo Jiang * 1 Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America, 2 College of Life Sciences, Peking University, Beijing, China, 3 Southern Medical University, Guangzhou, Guangdong, China, 4 Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, Guangzhou, China Abstract A novel swine-origin pandemic influenza A(H1N1) virus (H1N1pdm, also referred to as S-OIV) was identified as the causative agent of the 21st century’s first influenza pandemic, but molecular features conferring its ability of human-to-human transmission has not been identified. Here we compared the protein sequences of 2009 H1N1pdm strains with those causing other pandemics and the viruses isolated from humans, swines and avians, and then analyzed the mutation trend of the residues at the signature and non-signature positions, which are species- and non-species-associated, respectively, in the proteins of H1N1pdm during the pandemic of 2009. We confirmed that the host-specific genomic signatures of 2009 H1N1pdm, which are mainly swine-like, were highly identical to those of the 1918 H1N1pdm. During the short period of time when the pandemic alert level was raised from phase 4 to phase 6, one signature residue at the position of NP-100 mutated from valine to isoleucine. Four non-signature residues, at positions NA-91, NA-233, HA-206, and NS1-123, also changed during the epidemic in 2009. All these mutant residues, except that at NA-9