screening of supports for the immobilization of -glucosidase筛选为葡糖苷酶的固定化.pdfVIP

SAGE-Hindawi Access to Research Enzyme Research Volume 2011, Article ID 642460, 8 pages doi:10.4061/2011/642460 Research Article Screening of Supports for the Immobilization of β-Glucosidase Joelise de Alencar Figueira,1, 2, 3 Fernanda Furlan Gonc¸alves Dias,3 ´ 3 1, 2 Helia Harumi Sato, and Pedro Fernandes 1 Department of Bioengineering, Higher Technical Institute (IST), Avenida Rovisco Pais, 1049-001 Lisboa, Portugal 2 Centre for Biological and Chemical Engineering, Institute for Biotechnology and Bioengineering (IBB), IST, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal 3 Laboratory of Food Biochemistry, Department of Food Science, School of Food Engineering, University of Campinas-UNICAMP, Avenida Monteiro Lobato 80, C. P. 6121, 13083-862, Campinas, SP, Brazil Correspondence should be addressed to Pedro Fernandes, pedro.fernandes@ist.utl.pt Received 13 April 2011; Revised 13 July 2011; Accepted 13 July 2011 Academic Editor: J. Guisan Copyright © 2011 Joelise de Alencar Figueira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A set of supports were screened for the immobilization of a partially purified extract of β -glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catal