screening of bacterial strains for polygalacturonase activity its production by bacillus sphaericus (mtcc 7542)筛选菌株的聚半乳糖醛酸酶活动的生产杆菌sphaericus(mtcc 7542).pdfVIP

SAGE-Hindawi Access to Research Enzyme Research Volume 2010, Article ID 306785, 5 pages doi:10.4061/2010/306785 Research Article Screening of Bacterial Strains for Polygalacturonase Activity: Its Production by Bacillus sphaericus (MTCC 7542) Ranveer Singh Jayani,1, 2 Surendra Kumar Shukla,1 and Reena Gupta1 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India 2 Chromatin Biology Laboratory, National Centre for Cell Science (NCCS), Ganeshkhind, Pune 411 007, India Correspondence should be addressed to Reena Gupta, reenagupta.hpu@ Received 1 May 2010; Revised 19 August 2010; Accepted 1 October 2010 Academic Editor: Jose Miguel Palomo Copyright © 2010 Ranveer Singh Jayani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. At present almost all the pectinolytic enzymes used for industrial applications are produced by fungi. There are a few reports of pectinase production by bacterial strains. Therefore, in the present study, seventy-four bacterial strains, isolated from soil and rotten vegetable samples, were screened for polygalacturonase production. The strain PG-31, which gave maximum activity, was identified as Bacillus sphaericus (MTCC 7542). Maximal quantities of polygalacturonase were produced when a 16-hours-old inoculum was used at 7.5% (v/v) in production medium and incubated in shaking conditions (160 rpm) for 72 hours. The optimal temperature and pH for bacterial growth and polygalacturonase production were found to be 30◦C and 6.8, respectively. Maximum enzyme production resulted when citrus pectin was used as the carbon source at a concentra