severe maternal hyperglycemia exacerbates the development of insulin resistance and fatty liver in the offspring on high fat diet严重的孕产妇高血糖加重胰岛素抵抗的发展和后代高脂肪饮食脂肪肝.pdfVIP

Hindawi Publishing Corporation Experimental Diabetes Research Volume 2012, Article ID 254976, 8 pages doi:10.1155/2012/254976 Research Article Severe Maternal Hyperglycemia Exacerbates the Development of Insulin Resistance and Fatty Liver in the Offspring on High Fat Diet Yong Song,1, 2 Jibin Li,2 Yong Zhao,2 Qijuan Zhang,1, 2 Zhiguo Liu,1 Jingna Li,1 Xiaoyi Chen,1 Zhu Yang,1 Chao Yu,1 and Xiaoqiu Xiao1 1 Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China 2 Department of Nutrition and Food Hygiene, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China Correspondence should be addressed to Jibin Li, ljb21st@126.com and Xiaoqiu Xiao, bx.shaw@ Received 19 December 2011; Revised 5 February 2012; Accepted 16 February 2012 Academic Editor: Mark A. Yorek Copyright © 2012 Yong Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Adverse maternal environments may predispose the offspring to metabolic syndrome in adulthoods, but the underlying mechanism has not been fully understood. Methods. Maternal hyperglycemia was induced by streptozotocin (STZ) injection while control (CON) rats received citrate buffer. Litters were adjusted to eight pups per dam and then weaned to standard diet. Since 13 weeks old, a subset of offspring from STZ and CON dams were switched to high fat diet (HFD) for another 13 weeks. Glucose and insulin tolerance tests (GTT and ITT) and insulin secretion assay were performed; serum levels of lipids and leptin were measured. Hepatic fat accumulation and islet area were evaluated through haemat