soybean lectin enhances biofilm formation by bradyrhizobium japonicum in the absence of plants大豆凝集素增强bradyrhizobium日本血吸虫的生物膜的形成没有植物.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2009, Article ID 719367, 8 pages doi:10.1155/2009/719367 Research Article Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants ´ ´ ´ Julieta Perez-Gimenez, Elıas J. Mongiardini, M. Julia Althabegoiti, Julieta Covelli, ´ ´ ´ J. Ignacio Quelas, Silvina L. Lopez-Garcıa, and Anıbal R. Lodeiro Instituto de Biotecnologıa y Biologıa Molecular, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, ´ ´ ´ Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, Argentina ´ Correspondence should be addressed to Anıbal R. Lodeiro, lodeiro@.ar Received 13 August 2008; Revised 13 December 2008; Accepted 13 March 2009 Recommended by Dulal Borthakur Soybean lectin (SBL) purified from soybean seeds by affinity chromatography strongly bound to Bradyrhizobium japonicum USDA 110 cell surface. This lectin enhanced biofilm formation by B. japonicum in a concentration-dependent manner. Presence of galactose during biofilm formation had different effects in the presence or absence of SBL. Biofilms were completely inhibited in the presence of both SBL and galactose, while in the absence of SBL, galactose was less inhibitory. SBL was very stable, since its agglutinating activity of B. japonicum cells as well as of human group A+ erythrocytes was resistant to preincubation for one week at 60◦C. Hence, we propose that plant remnants might constitute a source of this lectin, which might remain act