secretome analysis of skeletal myogenesis using silac and shotgun proteomicssecretome骨胳肌细胞生成使用silac和猎枪蛋白质组学分析.pdfVIP

Hindawi Publishing Corporation International Journal of Proteomics Volume 2011, Article ID 329467, 13 pages doi:10.1155/2011/329467 Review Article Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics C. Y. X’avia Chan,1, 2 John C. McDermott,1, 2, 3, 4 and K. W. Michael Siu1, 2, 5 1 Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3 2 Centre for Research in Mass Spectrometry (CRMS), York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3 3 Muscle Health Research Centre (MHRC), York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3 4 Centre for Research in Biomolecular Interactions (CRBI), York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3 5 Department of Chemistry, York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3 Correspondence should be addressed to John C. McDermott, jmcderm@yorku.ca and K. W. Michael Siu, kwmsiu@yorku.ca Received 15 November 2010; Accepted 26 January 2011 ¨ Academic Editor: Uwe Volker Copyright © 2011 C. Y. X’avia Chan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Myogenesis, the formation of skeletal muscle, is a multistep event that commences with myoblast proliferation, followed by cell-cycle arrest, and finally the formation of multinucleated myotubes via fusion of mononucleated myoblasts. Each step is orchestrated by well-documented intracellular factors, such as cytoplasmic signalling molecules and nuclear transcription factors. Regardless, the key step in getting a more compre